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Splashed White Testing - Discussion with one of the researchers at UC Davis VGL

I was refered to one of the researchers at UC Davis VGL yesterday regarding one of the negative tests results on one of my horses. The horse Cross Country Blue Moon is listed on our thread of negative SW test results on this forum. Blue Moon phenotypically looks to be a tobiano splashed white combination. He was tested homozygous for tobiano, but negative for splashed white 1,2,and 3. She agreed that he looked to be carrying the splashed white gene and he has been tagged for further study. The highlights of the conversation are as follows: 1.) It is helpful for you to include a photo with the test request. This is done from your computer where it says "Optional Picture". You click on Manage Picture and choose a photo which shows your horse's color the best. A side view, like what you would use for registering a horse, is great. They do look at these photos. 2.) Once they run more tests they will know if the lethal form is found in other breeds, such as the miniature horse. 3.) Phenotypically, the lethal forms SW-2 and SW-3, do not look different from the SW-1. 4.) They are seeing a few (2-3 so far) horses that appear (phenotypically) to be Splashed White, yet are testing negative. They still feel there may be more Splashed White genes that have yet to be mapped. These horses are being tagged for future testing. If you feel that one of your horses may fall into this category, you can send in additional photos and the testing number to them for their review. It is helpful if you can document that you have tested the horse for other color genes and what those results were. 5.) Unfortunatly the test form does not ask if the horse has blue eyes, or brown eyes, or a mixture, so it is not yet know if there are brown-eyed splashed whites yet. These horses, if they exist, may be a combination of pinto patterns.

rodeoratdogs Sat, 02/04/2012 - 20:24

I didn't even know they had the test yet?! Where have I been? I should get Annie tested, is it just UC Davis that has the test so far?? Animal genetics already has Annie's DNA.

Threnody Sat, 02/04/2012 - 20:50

Thanks Pacific Pintos!

And where have you been rodeo? It's been the talk of the horse forums! It is just UC Davis who has the test at the moment. And please test!

rodeoratdogs Sun, 02/05/2012 - 09:20

In reply to by Daylene Alford

Woops :ymblushing: yeah I haven't been on any forums for awhile, ok yeah I'll look into getting her tested. Should be interesting. She was negative for LWO but she has really interesting "Splashy" markings on her face but the white doesn't touch her eye's so everybody on here was thinking she must be Splash/sabino.

So do I just go to the UC Davis website then to get the forms to fill out?

rodeoratdogs Sun, 02/05/2012 - 09:55

Thanks I'm filling our the form now, but you know where they ask for her color should I just put Dun?? It's not spacific?? ....oh ok I see the red dun choice now, it also has a Overo choice and that's what it says on her paper's but she is Negative for frame so are they looking for what her paper's say or are they asking if she is really Frame??

rodeoratdogs Sun, 02/05/2012 - 18:23

In reply to by Daylene Alford

[quote=Danni][quote=admin]Ya, mark her "Overo" I think what they are meaning is basically "not Tobiano". %-([/quote]

Oh I couldn't fill in overo just because a horse has splashy markings LOL, I don't care what UC Davic was meaning!![/quote]

LOL, yeah , I actually called today to clarify(waiting for a call back)....ok so you want me to mark this Overo box even if she's not?? Or should I say so do you want me to tell you the truth or do you want me to lie to ya?? :^O

rodeoratdogs Mon, 02/06/2012 - 10:43

Ok I saved my test and talked to one of the researchers and she said I should test for sabino 1 as well? She said that Sabino was in QH lines but I thought that most of you on here agreed that it wasn't...so should or shouldn't I test for sabino 1 as well????

critterkeeper Mon, 02/06/2012 - 18:27

I am not sure why anyone would think SB1 is not in QHs, as we all know, QH lines possess more than just a few TBs (like a whole bunch more... :rofl ) I only have one SB-1 positive and she is my Paint, Patches...but she is foundation bred QH lines (all crop outs in her pedigree: she is both SB1 and frame positive and looks very splashy w/o blue eyes).

rodeoratdogs Mon, 02/06/2012 - 19:19

In reply to by Daylene Alford

Hmmmmm well I could have swore somebody on here told me don't bother with Sabino 1 because it was more commonly found in like TWH and shetlands and not so much in QH's. It's probably even in a old thread somewhere. That being said I called Animal genetics today since they already have Annie's DNA to ask when they would start testing for SW. They said they expect to be starting testing for SW in 4-6 weeks and they would really like to test Annie because they need test subjects, so the test would be free and they will throw in in the Sabino 1 test for free as well :D Bonus \\\\:d/

rodeoratdogs Thu, 02/09/2012 - 17:33

K nevermind, I talked to Arne again at Animal geneitics and he wasn't sure when they would get the tests and if they would get all 3 Sw1, Sw2 and 3 so I sent her sample off to UC Davis today. He said he wanted me to let him know the results though.

rodeoratdogs Thu, 02/16/2012 - 13:48

YES!! :bounce thought so, here is Annie's test result from UC Davis...

Coat Color results for BDB FASHION DESIGN(DT29557):

Splashed White SW-1 Result:
N/SW1 - Horse has one copy of the SW-1 mutation.

Splashed White SW-2 Result:
N/N - No copies of SW-2 mutation.

Splashed White SW-3 Result:
N/N - No copies of SW-3 mutation.

Danni Wed, 02/22/2012 - 04:15

Very cool, I'm glad some are testing positive!! Did anyone see the line of splash whites in Australia that are all negative for SW?

Ameria Park Mi… Wed, 02/22/2012 - 19:51

Im from australia, have looked the horses that have tested Neg for splash white, IMO they all look Sabino/overo they have uneven edges on there white, not flat like a true splash, so maybe they arent splash??

when i look at a true splash they have bottom heavy blazes, flat top white markings, and look like they have been dipped in paint, these horses have more uneven markings with pointy edges like sabinos...its only my opinion... :grin:

Danni Thu, 02/23/2012 - 02:52

These are some of the splashed whites that are negative

[img]http://a7.sphotos.ak.fbcdn.net/hphotos-…]

Hind view of above horse
[img]http://a8.sphotos.ak.fbcdn.net/hphotos-…]

[img]http://a5.sphotos.ak.fbcdn.net/hphotos-…] [img]http://a4.sphotos.ak.fbcdn.net/hphotos-…]

[img]http://a2.sphotos.ak.fbcdn.net/hphotos-…] [img]http://a6.sphotos.ak.fbcdn.net/hphotos-…]

Some of the foals are by this negative SW stallion, who consistantly throws very splash markings, as well as the more sabino ones. He's bred on the same line as the first horse, he is frame overo negative. He did test positive for sb1 at animal genetics a year or two ago, but negative for sb1 recently at UC Davis. So maybe the test is better now?!

[img]http://a2.sphotos.ak.fbcdn.net/hphotos-…] [img]http://a1.sphotos.ak.fbcdn.net/hphotos-…]

There is no doubt in my mind that these horses are splashed white, some even are deaf. So the SW test seems only good for some lines so far

lillith Thu, 02/23/2012 - 07:49

In reply to by Daylene Alford

Lovely looking horse Danni - who is he? (curious me) I definitely agree the he looks what I would have called 'classic' high expression splash before the test came out.

Pacific Pintos Thu, 02/23/2012 - 08:11

In reply to by Daylene Alford

There have been many miniature horses that have tested negative that have already been tested for sabino and are negative and they appear to have classic splashed white markings.

Here are two examples of our negative sabino and splashed white; also negative for LWO BTW. Positive for Tobiano.

Bright Blue Eyes on this one:

[img]http://pacificpintos.com/referencesires…] [img]http://pacificpintos.com/referencesires…]

Partial Blues on this one:

[img]http://pacificpintos.com/broodmares/ima…] [img]http://pacificpintos.com/broodmares/ima…]

Both these horses have been thrown back into the research to see if there is an additional splashed white gene classification.

Danni Thu, 02/23/2012 - 08:14

The last stallion? He's at Dun Splashin Stud, where they breed splashed white paints, and all turned out negative LOL. His name is Anasazi Windtalker

thorwood Fri, 02/24/2012 - 00:48

LOL, & to add to the discussion, most of the "slpashy" Dunsplashin paints are deaf.
Also frame neg.